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Narrative

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Source1

{
  "resourceType": "PlanDefinition",
  "id": "mii-exa-molgen-protocol-agilent-sureselect",
  "text": {
    "status": "extensions",
    "div": "<!-- snip (see above) -->"
  },
  "url": "https://www.medizininformatik-initiative.de/fhir/ext/modul-molgen/PlanDefinition/mii-exa-molgen-protocol-agilent-sureselect",
  "version": "2027.0.0-ballot.1",
  "name": "AgilentSureSelectV7",
  "title": "Agilent SureSelect Human All Exon V7 Kit Protocol",
  "type": {
    "coding": [
      {
        "system": "http://terminology.hl7.org/CodeSystem/plan-definition-type",
        "code": "protocol",
        "display": "Protocol"
      }
    ]
  },
  "status": "active",
  "date": "2026-09-13T16:58:02+00:00",
  "publisher": "Medizininformatik-Initiative",
  "contact": [
    {
      "name": "Medizininformatik-Initiative",
      "telecom": [
        {
          "system": "url",
          "value": "https://www.medizininformatik-initiative.de/"
        },
        {
          "system": "email",
          "value": "info@medizininformatik-initiative.de"
        }
      ]
    }
  ],
  "description": "Target enrichment protocol for whole exome sequencing covering 35.8 Mb of the human exome",
  "jurisdiction": [
    {
      "coding": [
        {
          "system": "urn:iso:std:iso:3166",
          "code": "DE",
          "display": "Germany"
        }
      ]
    }
  ],
  "action": [
    {
      "title": "DNA Fragmentation",
      "description": "Fragment genomic DNA to 150-200bp using Covaris"
    },
    {
      "title": "Library Preparation",
      "description": "End repair, A-tailing, adapter ligation"
    },
    {
      "title": "Hybridization",
      "description": "Hybridize with SureSelect baits for 24 hours at 65°C"
    },
    {
      "title": "Capture",
      "description": "Streptavidin bead capture and washing"
    },
    {
      "title": "Amplification",
      "description": "PCR amplification of captured library"
    }
  ]
}